Description

For in vitro determination of HDL – Cholesterol in serum or plasma.

State	Liquid
Storage temp.	+2 / +8 ºC
Technique	Colorimetric method

PRINCIPLE

HDL- cholesterol direct is used for the determination of this cholesterol fraction, with no previous treatment, that is to say, without precipitation nor centrifugation.

The method is based on the property of a detergent to liberate the HDL fraction by solubilization, which then reacts with the chromogen, cholesterol esterase and oxidase to give rise to a measurement color at 600nm. The use of a polyanion stabilizes the lipoproteins (VLDL, LDL and chylomicrons) by adsorption, and therefore cannot react with the enzymatic complex.

DIAGNOSTIC USE

The fraction of the cholesterol bound to high density lipoprotein is an indicator of the risk of coronary heart disease. High levels of HDL-cholesterol appear to act as a protective factor, while low values are one of the major risk factors. Determination of HDL- cholesterol, along with comprehensive study of the lipid profile of the patient, allows assessing the risk of coronary heart disease. Low levels of HDL- cholesterol are found in cases of unbalanced diet, sedentary lifestyle, alcoholism or smoking.

A single test result could not be used to make a clinical diagnosis. It should integrate clinical and laboratory data.