Description

Calprotectin latex turbidimetric assay

INTENDED USE

Calprotectin Turbilatex, is a latex turbidimetric assay only for the quantitative detection
of calprotectin in human stool samples (not to be used for body fluid as blood, serum,
plasma, urine, cerebrospinal fluid, oral fluid, synovial fluid or empyema fluid).
This assay is simple and widely applicable. This product is optimized for several automated
analyser.
For professional in vitro diagnostic use only

PRINCIPLE

Calprotectin Turbilatex® is a latex turbidimetric assay only for the quantitative detection
of Calprotectin in human stool samples. This assay is simple, widely applicable and test
results are obtained in a very short time.
The intended use of the test is exclusively to differentiate IBD patients with inflammation
from IBD patients without inflammation and from irritable bowel syndrome (IBS).
Calprotectin latex turbidimetric assay is based on agglutination reactions. These involve
in vitro aggregation of microscopic latex particles. This aggregation consists in the
specific reaction between antigen and antibodies, antigen contained in the sample and
the antibodies anti-antigen coated on polystyrene latex particles. The sample is mixed
with a suspension containing antibodies against the antigen bound to latex particles. If
antigen is present in the sample, it will react with the antibodies and form an aggregate.
If no antigen is present in the sample the mixture will keep its appearance as a smooth
suspension. Such turbidity is measured as an increase in absorbance at determinate
wavelength and is proportional to the quantity of antigen contained in the sample.